random hexamer primers (Qiagen)

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Qiagen random hexamer primers
Random Hexamer Primers, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 2503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+primers/Random+Hexamers/pm42068851-57-30-35
Average 97 stars, based on 2503 article reviews

random hexamer primers - by Bioz Stars, 2026-09

97/100 stars

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cDNA Synthesis:

Article Title: Sex differences in endurance exercise capacity and skeletal muscle lipid metabolism in mice.
Article Snippet: .. In brief, RNA was extracted from the heart, quadriceps, and liver tissues using the RNeasy Mini Kit (#74104, Qiagen, Hilden, Germany). cDNA synthesis was performed using Omniscript reverse transcriptase (RT) kit and random primers according to the manufacturer's instructions (#205111, Qiagen, Hilden, Germany). .. RT- PCR was performed using SYBRGreen (Bio- Rad, Hercules, CA) and cDNA transcripts were quantified using a relative standard curve.

Article Title: Human macrophage polarization shapes B. pertussis intracellular persistence.
Article Snippet: Synthesis of cDNAwas performedwith theM-MLVReverse Transcriptase (Promega, Madison, WI, USA). .. For each cDNA synthesis, 40 ng of a given total RNA and 10 μM random primers (Qiagen, Germantown, MD, USA) were used as previously described.26 Complementary DNA samples were stored at−20◦C until the time of analyses by the PCR. .. Quantitative real-time PCR (qRT-PCR) analyses were performed on an Mx3000P QPCR System (Stratagene, La Jolla, CA, USA) with the SYBR Green PCR Master Mix (Roche, Mannheim, Germany) and 0.5 μM of each forward and reverse primer.

Article Title: Bordetella parapertussis adenylate cyclase toxin promotes the bacterial survival to the encounter with macrophages.
Article Snippet: B. parapertussis is a whooping cough etiological agent, whose incidence in the population has increased remarkably.. Virulence factors involved in the bacterial infection, however, remain poorly investigated.. We here studied the role of adenylate cyclase (CyaA), the main toxin of B. parapertussis, in the outcome of the bacterial interaction with macrophages.

Reverse Transcription:

Article Title: Comprehensive investigation and regulatory function of lncRNAs engaged in western honey bee larval immune response to Ascosphaera apis invasion
Article Snippet: The total RNA of the gut samples in the aforementioned six groups was extracted using the TRIzol method (Promega, United States). .. Oligo (dTs) was used to isolate poly (A) mRNA, which was subsequently fragmented and reverse transcribed using random primers (QIAGEN, Germany). .. Next, second-strand cDNA was synthesized with RNase H and DNA polymerase I, and the double-strand cDNA was then purified by the QiaQuick PCR extraction kit (QIAGEN, Germany).

Article Title: Lsh Mediated RNA Polymerase II Stalling at HoxC6 and HoxC8 Involves DNA Methylation
Article Snippet: The following antibodies were used: normal mouse or rabbit IgG (Upstate), rabbit anti-Lsh recombinant protein affinity-purified antibody ; 8WG16 Pol II (ab817), H14 Pol II (ab24759), H5 Pol II (ab24758, anti-H3 (ab1791), anti-H3 (di methyl K79) (ab3594), anti-H3 (tri methyl K36) (ab9050), anti-H3 (tri methyl K4) (ab8580) (Abcam); 5-Methylcytidine (BI-MECY-0100)(Eurogentec); anti-Flag (F1804), anti-β-actin (A2228), anti-Brdu (B2531)(Sigma); anti-Dnmt3b (ALX-804-233-C100)(Alexis Biochemicals); anti-CHD1 (NB100-60411) (Novus Biologicals Inc). .. For RT-PCR analysis total RNA was extracted using Qiagen RNeasy Mini Kit and 2 μg were reverse transcribed with 50 ng random primers and 10U Reverse Transcriptase (Superscript III Kit, Invitrogen). ..

Article Title: Novel Doublesex Duplication Associated with Sexually Dimorphic Development of Dogface Butterfly Wings
Article Snippet: .. We dissected the following tissues and extracted RNA for 3–6 males and females: adult head, adult thorax, adult abdomen, adult gonads and fifth instar larval gonadal masses. mRNA was extracted with a Qiagen Total RNA isolation kit, and cDNA was synthesized using random primers and reverse transcriptase. ..

Article Title: Enteric viruses in lentic and lotic freshwater habitats from Brazil's Midwest and South regions in the Guarani Aquifer area.
Article Snippet: The present study reports the monitoring of viruses indicating fecal contamination in two distinct regions affected by poor management of wastewater located above the Guarani Aquifer, which is one of the biggest freshwater reservoirs in the world.. In the city of Três Lagoas (located in the Midwest region, in the state of Mato Grosso do Sul), water samples were collected from Lagoa Maior, a lake used for recreation, and in Concórdia (located in the South region, in the state of Santa Catarina), from the Queimados River, which crosses the urban area.. Four sampling sites were monitored from March to July 2018 in Lagoa Maior, and four sampling sites were monitored along the urban part of the Queimados River area over two periods (rainy and dry).Water samples were analyzed by concentration ofHuman adenovirus (HAdV), Norovirus (NoV), Rotavirus A (RAV), and Hepatitis A virus (HAV) for the Lagoa Maior samples and RVA, HAV, and Porcine circovirus 2 (PCV2) for the Queimados River samples.

Reverse Transcription Polymerase Chain Reaction:

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Article Title: Alcohol extract of the gypsy mushroom (Cortinarius caperatus) inhibits the development of Deformed wing virus infection in western honey bee (Apis mellifera).
Article Snippet: Deformed wing virus (DWV) transmitted by the parasitic mite Varroa destructor is one of the most significant factors contributing to massive losses of managed colonies of western honey bee (Apis mellifera) subspecies of European origin reported worldwide in recent decades.. Despite this fact, no antiviral treatment against honey bee viruses is currently available for practical applications and the level of viral infection can only be controlled indirectly by reducing the number of Varroa mites in honey bee colonies.. In this study, we investigated the antiviral potential of the gypsy mushroom (Cortinarius caperatus) to reduce DWV infection in honey bees.

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